Journal:

Article Title: Schistosomal egg antigen-responsive CD8 T-cell population in Schistosoma mansoni -infected BALB/c mice

doi: 10.1046/j.1365-2567.1999.00887.x

Figure Lengend Snippet: Cytokine production after in vitro stimulation of CD8+ T cells from SEACNBr‐immunized mice. CD8+ T cells were isolated from spleens removed 21 days after immunization of BALB/c mice with 50 µg of SEACNBr. IL‐2, IL‐4, IL‐5, IL‐10 and IFN‐γ were measured by specific ELISA in cell‐culture supernatants after 24 hr of stimulation with Con A (10 µg/ml) or anti‐CD3 antibody (5 µg/ml) or 96 hr of stimulation with SEACNBr (50 µg/ml). Results are expressed as mean ± SEM.

Article Snippet: CD8 + T cells were separated by the magnetic cell sorting (MACS) method using colloidal super‐magnetic microbeads conjugated with monoclonal rat anti‐mouse CD8a (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer's instructions.

Techniques: In Vitro, Isolation, Enzyme-linked Immunosorbent Assay, Cell Culture

Journal:

Article Title: Influence of Glycosylation on the Efficacy of an Env-Based Vaccine against Simian Immunodeficiency Virus SIVmac239 in a Macaque AIDS Model

doi: 10.1128/JVI.79.16.10386-10396.2005

Figure Lengend Snippet: Env-specific CD4+ T-cell and CD8+ T-cell responses in 12 macaques. A: Env-specific CD8+ T cells in PBMCs were measured by ELISPOT assay for IFN-γ in three groups. B: Env-specific CD4+ T cells in PBMCs were measured by ELISPOT assay for IFN-γ in three groups. ELISPOT results are colored as follows: Δ5G SU-specific T cells (red), wt-SU-specific T cells (green), and TM-specific T cells (yellow). Arrows with a dotted line, arrows with broken line, and arrows with a solid line indicate the time of the third DNA vaccination at 8 weeks p.p., the time of the vaccine boost at 21 weeks p.p., and the time of SIVmac239 challenge at 28 weeks p.p., respectively. C: Comparison of SU-specific CD8+ T cells and CD4+ T cells in PBMCs among the wt-Env vaccine group, the Δ5G Env vaccine group, and the vector control group at 26 weeks p.p. and 4, 7, and 12 days p.i. The numbers of SFC responding to SIVmac239 SU were used to compare the effects of the two vaccines. w, weeks; d, days.

Article Snippet: PBMCs were subjected to the depletion of CD4 + cells with magnet beads coated with anti-human CD4 Ab (Dynal ASA, Oslo, Norway) or subjected to the depletion of CD8 + cells with magnet beads coated with anti-human CD8 Ab (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Enzyme-linked Immunospot, Comparison, Plasmid Preparation, Vaccines

Journal:

Article Title: Influence of Glycosylation on the Efficacy of an Env-Based Vaccine against Simian Immunodeficiency Virus SIVmac239 in a Macaque AIDS Model

doi: 10.1128/JVI.79.16.10386-10396.2005

Figure Lengend Snippet: SIV-specific CD8+ T-cell and CD4+ T-cell responses in 12 animals. A: SIV viral-protein-specific CD8+ T cells in PBMCs were measured by ELISPOT assay for IFN-γ in three groups: vector controls, wt-Env vaccine group, and Δ5G Env vaccines. B: SIV viral-protein-specific CD4+ T cells in PBMCs were measured by ELISPOT assay for IFN-γ in three groups. ELISPOT results of individual SIV proteins are colored as follows: Gag (red), Nef (green), Tat/Rev (blue), Vif/Vpr/Vpx (yellow), and Pol (pink). C: Comparison of cumulated CD8+ T cells or CD4+ T cells specific to the viral proteins Gag, Pol, Nef, Tat/Rev, and Vif/Vpr/VpX between SIV infection-controlled and uncontrolled animals. w, weeks; d, days.

Article Snippet: PBMCs were subjected to the depletion of CD4 + cells with magnet beads coated with anti-human CD4 Ab (Dynal ASA, Oslo, Norway) or subjected to the depletion of CD8 + cells with magnet beads coated with anti-human CD8 Ab (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Enzyme-linked Immunospot, Plasmid Preparation, Vaccines, Comparison, Infection

Journal: Materials Today Bio

Article Title: A pH/MMP-9 smart dual-responsive liposome GBE@LP co-delivers and controls the release of GB1107/BMS1166/Enzalutamide for liver cancer immunotherapy

doi: 10.1016/j.mtbio.2025.101801

Figure Lengend Snippet: Graphical illustration of the immunization strategy of GBE@LP for liver cancer treatment. GBE@LP has prolonged circulation in the bloodstream due to surface PEG and selectively accumulates in liver cancer tissues through EPR effect. Meanwhile, DOPE and TGMS give it the property to be released in response to low pH and MMP-9 overexpression in liver cancer microenvironment. GBE@LP delivers drugs into liver cancer, converting uninfiltrated "cold" tumors into highly infiltrated "hot" tumors, while reversing CD8 + T-cell depletion and enhancing cytotoxicity. These effects ultimately enhance immunotherapy for liver cancer.

Article Snippet: After deparaffinization and rehydration, the sections were microwaved in 0.01M sodium citrate (pH 6) for 8 min, followed by blocking with 5 % bovine serum albumin (BSA) for 1 h. The sections were then incubated with anti-mouse CD8, anti-mouse PD-1, and anti-mouse AR antibodies (all from Proteintech) at 4 °C for 12 h. After washing, the sections were incubated with Dylight 649 (Goat Anti-Mouse IgG) and Dylight 488 (Goat Anti-Rabbit IgG) antibodies (all from Abbkine) for 1 h, stained with DAPI for 5 min, and then mounted.

Techniques: Over Expression

Journal: Materials Today Bio

Article Title: A pH/MMP-9 smart dual-responsive liposome GBE@LP co-delivers and controls the release of GB1107/BMS1166/Enzalutamide for liver cancer immunotherapy

doi: 10.1016/j.mtbio.2025.101801

Figure Lengend Snippet: Liver cancer is a "cold" tumor with high Gal-3 expression. (A) Immunofluorescence images of CD8 + T cells (red) and DAPI (blue) in liver cancer tissues of tumor-bearing mice. (B) Immunohistochemical images of cd8 + T cells in patients with hepatocellular carcinoma (HCC), renal cell carcinoma (RCC), and lung cancer (LC) from the HPA database. (C) Expression of Gal-3 in different human cancers was analyzed by HPA database. (D) The expression of Gal-3 in mouse liver cancer tissues and paracancerous tissues was detected by western blotting. (E) The expression of CD8 in the control group and GB1107 treatment group was detected by western blotting. (F) Immunofluorescence images of CD8 + T cells (green), PD-1 (red), and DAPI (blue) in liver cancer tissues and paracancerous tissues of tumor-bearing mice. (G) Immunofluorescence images of CD8 + T cells (green), AR (red), and DAPI (blue) in liver cancer tissues and paracancerous tissues of tumor-bearing mice.

Article Snippet: After deparaffinization and rehydration, the sections were microwaved in 0.01M sodium citrate (pH 6) for 8 min, followed by blocking with 5 % bovine serum albumin (BSA) for 1 h. The sections were then incubated with anti-mouse CD8, anti-mouse PD-1, and anti-mouse AR antibodies (all from Proteintech) at 4 °C for 12 h. After washing, the sections were incubated with Dylight 649 (Goat Anti-Mouse IgG) and Dylight 488 (Goat Anti-Rabbit IgG) antibodies (all from Abbkine) for 1 h, stained with DAPI for 5 min, and then mounted.

Techniques: Expressing, Immunofluorescence, Immunohistochemical staining, Western Blot, Control

Journal: Materials Today Bio

Article Title: A pH/MMP-9 smart dual-responsive liposome GBE@LP co-delivers and controls the release of GB1107/BMS1166/Enzalutamide for liver cancer immunotherapy

doi: 10.1016/j.mtbio.2025.101801

Figure Lengend Snippet: GBE@LP remodeling the immunosuppressive microenvironment of liver cancer. (A) Flow cytometry scatter graph and (C) Flow cytometry statistical plot of CD8 + T cells infiltration in the tumors of different treatment groups. (B) Flow cytometry histogram and (D) Flow cytometry statistical plot of CD3 + T cells infiltration in the spleen of different treatment groups. (n = 3, p ∗ < 0.05, p ∗∗ < 0.01, p ∗∗∗ < 0.001, p ∗∗∗∗ < 0.0001).

Article Snippet: After deparaffinization and rehydration, the sections were microwaved in 0.01M sodium citrate (pH 6) for 8 min, followed by blocking with 5 % bovine serum albumin (BSA) for 1 h. The sections were then incubated with anti-mouse CD8, anti-mouse PD-1, and anti-mouse AR antibodies (all from Proteintech) at 4 °C for 12 h. After washing, the sections were incubated with Dylight 649 (Goat Anti-Mouse IgG) and Dylight 488 (Goat Anti-Rabbit IgG) antibodies (all from Abbkine) for 1 h, stained with DAPI for 5 min, and then mounted.

Techniques: Flow Cytometry

Journal: Materials Today Bio

Article Title: A pH/MMP-9 smart dual-responsive liposome GBE@LP co-delivers and controls the release of GB1107/BMS1166/Enzalutamide for liver cancer immunotherapy

doi: 10.1016/j.mtbio.2025.101801

Figure Lengend Snippet: (A) Immunofluorescence images of CD8 + T cells (green) and DAPI (blue) in different treatment groups. Scale bars, 1000 μm (top), 50 μm (bottom).

Article Snippet: After deparaffinization and rehydration, the sections were microwaved in 0.01M sodium citrate (pH 6) for 8 min, followed by blocking with 5 % bovine serum albumin (BSA) for 1 h. The sections were then incubated with anti-mouse CD8, anti-mouse PD-1, and anti-mouse AR antibodies (all from Proteintech) at 4 °C for 12 h. After washing, the sections were incubated with Dylight 649 (Goat Anti-Mouse IgG) and Dylight 488 (Goat Anti-Rabbit IgG) antibodies (all from Abbkine) for 1 h, stained with DAPI for 5 min, and then mounted.

Techniques: Immunofluorescence

Journal: Asian Journal of Pharmaceutical Sciences

Article Title: Lignin-assisted construction of sub-10 nm supramolecular self-assembly for photothermal immunotherapy and potentiating anti-PD-1 therapy against primary and distant breast tumors

doi: 10.1016/j.ajps.2022.07.002

Figure Lengend Snippet: In vivo immune responses assessment. (A) Flow cytometry assay of CD80 + or CD86 + gated on CD11c + in the TDLNs of mice. (B) Flow cytometry assay illustrated the percent of CD8a + T cells and CD4 + T cells gated on CD3 + T cells in splenocytes of mice. (C-D) Quantitative percentages of CD80 + or CD86 + expressions gated on CD11c + according to (A). (E) Quantitative percentages of CD8a + T cells and CD4 + T cells in splenocytes according to (B). (F-H) Quantitative analysis of the immune-associated cytokines in serum, as determined by ELISA (IL-2, TNF-α and IFN-γ). ( n = 3, mean ± SD, * P < 0.05, ** P < 0.01 and ## P < 0.0001).

Article Snippet: Fluorochrome-labeled anti-mouse monoclonal antibodies (CD11c-allophycocyanin (APC), CD80-fluorescein isothiocyanate (FITC), CD86-phycoerythrin (PE), CD3-FITC, CD8a-PE, and CD4-APC) were bought from Proteintech.

Techniques: In Vivo, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Mitigation of Tacrolimus-Associated Nephrotoxicity by PLGA Nanoparticulate Delivery Following Multiple Dosing to Mice while Maintaining its Immunosuppressive Activity

doi: 10.1038/s41598-020-63767-1

Figure Lengend Snippet: In vivo suppression of T cell proliferation in male mice following a daily subcutaneous injection (for 7 days) of either (1) Normal Saline, (2) Empty PLGA NPs, (3) Prograf, or (4) TAC-loaded PLGA NPs. Flow cytometry analysis was conducted on two cell groups: ( A ) CD4 + T cells and ( B ) CD8 + T cells (n = 6). The percentage of proliferating T cells is presented next to each gate. All samples are composed of the same number of acquired events (10 6 cells).

Article Snippet: Standard surface staining protocol was followed for CD4 + and CD8 + T cells using anti-mouse CD4–APC (R&D systems) and CD8-PE-CY7 (R&D systems) monoclonal antibodies.

Techniques: In Vivo, Injection, Saline, Flow Cytometry